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miR-706 tiggers ER stress dependent cell death trough <t>CAAP1.</t> A. Target scan prediction showing predicted binding of miR-706 and CAAP1 3ˊUTR. B. NIH3T3 cells were transfected with scrambled microRNA (control; ctrl) or mimic miR-706 (mimic). Total RNA was purified and CAAP1 expression was determined using qRT-PCR. C. NIH3T3 cells were transduced with antisense miR-706 (anti-706) and then incubated with Thapsigargin for 24 hours. Total RNA was purified and CAAP1 expression was determined using qRT-PCR. D. NIH3T3 cells were transduced with scrambled microRNA (ctrl), antisense miR-706 (anti-706) or antisense miR-706 and shCAAP1. The cells were then incubated with Thapsigargin for 24 hours. Cell death was assesed by Annexin V staining. E. Quantification of panel C. *; P<0.05, **; P<0.01 compared to control, and qRT-PCR; Quantitative reverse transcription polymerase chain reaction.

Caap1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/caap1/product/Novus Biologicals
Average 90 stars, based on 2 article reviews

caap1 - by Bioz Stars, 2026-05

90/100 stars

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1) Product Images from "Endoplasmic Reticulum Stress Induces miR-706, A Pro-Cell Death microRNA, in A Protein Kinase RNA-Like ER Kinase (PERK) and Activating Transcription Factor 4 (ATF4) Dependent Manner"

Article Title: Endoplasmic Reticulum Stress Induces miR-706, A Pro-Cell Death microRNA, in A Protein Kinase RNA-Like ER Kinase (PERK) and Activating Transcription Factor 4 (ATF4) Dependent Manner

Journal: Cell Journal (Yakhteh)

doi: 10.22074/cellj.2020.6873

miR-706 tiggers ER stress dependent cell death trough CAAP1. A. Target scan prediction showing predicted binding of miR-706 and CAAP1 3ˊUTR. B. NIH3T3 cells were transfected with scrambled microRNA (control; ctrl) or mimic miR-706 (mimic). Total RNA was purified and CAAP1 expression was determined using qRT-PCR. C. NIH3T3 cells were transduced with antisense miR-706 (anti-706) and then incubated with Thapsigargin for 24 hours. Total RNA was purified and CAAP1 expression was determined using qRT-PCR. D. NIH3T3 cells were transduced with scrambled microRNA (ctrl), antisense miR-706 (anti-706) or antisense miR-706 and shCAAP1. The cells were then incubated with Thapsigargin for 24 hours. Cell death was assesed by Annexin V staining. E. Quantification of panel C. *; P<0.05, **; P<0.01 compared to control, and qRT-PCR; Quantitative reverse transcription polymerase chain reaction.

Figure Legend Snippet: miR-706 tiggers ER stress dependent cell death trough CAAP1. A. Target scan prediction showing predicted binding of miR-706 and CAAP1 3ˊUTR. B. NIH3T3 cells were transfected with scrambled microRNA (control; ctrl) or mimic miR-706 (mimic). Total RNA was purified and CAAP1 expression was determined using qRT-PCR. C. NIH3T3 cells were transduced with antisense miR-706 (anti-706) and then incubated with Thapsigargin for 24 hours. Total RNA was purified and CAAP1 expression was determined using qRT-PCR. D. NIH3T3 cells were transduced with scrambled microRNA (ctrl), antisense miR-706 (anti-706) or antisense miR-706 and shCAAP1. The cells were then incubated with Thapsigargin for 24 hours. Cell death was assesed by Annexin V staining. E. Quantification of panel C. *; P<0.05, **; P<0.01 compared to control, and qRT-PCR; Quantitative reverse transcription polymerase chain reaction.

Techniques Used: Binding Assay, Transfection, Control, Purification, Expressing, Quantitative RT-PCR, Transduction, Incubation, Staining, Reverse Transcription, Polymerase Chain Reaction

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CAAP1 and BCL2L2-PABPN can promote cellular proliferation. (A) Western blot analysis of total cell lysates of HNSCC cell lines demonstrates the expression of CAAP1 and BCL2L2-PABPN. (B) CAAP1 and BCL2L2-PABPN expressions in five paired tumor tissues (T) and their adjacent normal tissues (N). (C) The expression of CAAP1 and BCL2L2-PABPN in FADU and HN8 cells respectively after transferring with sgRNA. Knockdown of CAAP1 and BCL2L2-PABPN inhibited the Invasion and migration of HNSCC cells were test by wound-healing assay (D), transwell assay (E).

Journal: American Journal of Cancer Research

Article Title: Panoramic analysis of cell death patterns reveals prognostic and immune profiles of head and neck squamous cell carcinoma

doi: 10.62347/PMDA6193

Figure Lengend Snippet: CAAP1 and BCL2L2-PABPN can promote cellular proliferation. (A) Western blot analysis of total cell lysates of HNSCC cell lines demonstrates the expression of CAAP1 and BCL2L2-PABPN. (B) CAAP1 and BCL2L2-PABPN expressions in five paired tumor tissues (T) and their adjacent normal tissues (N). (C) The expression of CAAP1 and BCL2L2-PABPN in FADU and HN8 cells respectively after transferring with sgRNA. Knockdown of CAAP1 and BCL2L2-PABPN inhibited the Invasion and migration of HNSCC cells were test by wound-healing assay (D), transwell assay (E).

Article Snippet: For the purpose of immunoblotting, the following antibodies were employed at a dilution of 1:1000: rabbit anti-CAAP1 antibody (PA5-54977, Thermofisher), rabbit anti-BCL2L2-PABPN1 antibody (NBP2-61706, NOVUS), rabbit anti-caspase 8 antibody (9746S, CST), rabbit anti-caspase 9 antibody (10380-AP, proteintech) and rabbit anti-Vinculin antibody (ab129002, Abcam); and mouse anti-GAPDH (60004-1-Ig, Proteintech).

Techniques: Western Blot, Expressing, Transferring, Migration, Wound Healing Assay, Transwell Assay

CAAP1 inhibit HNSCC intrinsic apoptosis, BCL2L2-PABPN inhibit HNSCC extrinsic apoptosis. (A) Phase-contrast photomicrographs showing Fadu cells treated with ABT-737 for 12 h, analysis with Cell Counting Kit CCK-8 assay (B). (C) Fadu and CAAP1-/- cells were treated with ABT-737 for 10 h, and then stained with Annexin V-FITC and analysed by flow cytometry; Percentage of apoptotic cells was measured right. (D) Western blot of full-length and cleaved caspase 9 for Fadu cell lines treated with ABT-737 (30 μM) for 12 h, (E) Bax level as measured using EIA assay. (F) Cells were photographed 24 h after drug treatment and analysis by CCK8 (G). (H) HN8 and BCL2L2-PABPN-/- cells were treated with TNF-α/CHX for 12 h, and then stained with Annexin V-FITC and analysed by flow cytometry; Percentage of apoptotic cells was measured right. CTRL, control. (I) Western blot of HN8 and BCL2L2-PABPN-/- cell lines treated with TNF-α and cycloheximide (CHX) for 12 h. TNF-α (5 ng/ml), CHX (20 ug/ml). (J) Caspase 8 activity as measured using enzymatic assay and represented by optical density at 450 nm (OD450).

Journal: American Journal of Cancer Research

Article Title: Panoramic analysis of cell death patterns reveals prognostic and immune profiles of head and neck squamous cell carcinoma

doi: 10.62347/PMDA6193

Figure Lengend Snippet: CAAP1 inhibit HNSCC intrinsic apoptosis, BCL2L2-PABPN inhibit HNSCC extrinsic apoptosis. (A) Phase-contrast photomicrographs showing Fadu cells treated with ABT-737 for 12 h, analysis with Cell Counting Kit CCK-8 assay (B). (C) Fadu and CAAP1-/- cells were treated with ABT-737 for 10 h, and then stained with Annexin V-FITC and analysed by flow cytometry; Percentage of apoptotic cells was measured right. (D) Western blot of full-length and cleaved caspase 9 for Fadu cell lines treated with ABT-737 (30 μM) for 12 h, (E) Bax level as measured using EIA assay. (F) Cells were photographed 24 h after drug treatment and analysis by CCK8 (G). (H) HN8 and BCL2L2-PABPN-/- cells were treated with TNF-α/CHX for 12 h, and then stained with Annexin V-FITC and analysed by flow cytometry; Percentage of apoptotic cells was measured right. CTRL, control. (I) Western blot of HN8 and BCL2L2-PABPN-/- cell lines treated with TNF-α and cycloheximide (CHX) for 12 h. TNF-α (5 ng/ml), CHX (20 ug/ml). (J) Caspase 8 activity as measured using enzymatic assay and represented by optical density at 450 nm (OD450).

Techniques: Cell Counting, CCK-8 Assay, Staining, Flow Cytometry, Western Blot, Enzyme Immunoassay, Activity Assay, Enzymatic Assay

In immune-competent mouse models, inhibition of CAAP1 induces antitumor immunity. (A) A schematic view of the treatment plan. (B) Tumor bodies dissected 15 days after tumor formation in BLAB/C nude mice. (C, D) Summary of tumor weight (C) and volume data (D) of Fadu tumors harvested after euthanizing the mice. (E) A schematic view of the treatment plan and C57 mice received GFP-Luc sg nc, or sg caap1 meer cells were injected with luciferin. (F, G) Summary of luciferase activity (F) and volume data (G) of meer tumors harvested after euthanizing the mice. (H) Differences in the expression of CAAP1, Bax, and Bak in tumor tissues among different groups. (I-K) FACS of CD8+ in CD3+ cells and GZMB+CD8+ in CD3+ TILs from sg nc, or sg caap1 meer xenografts and quantification.

Journal: American Journal of Cancer Research

Article Title: Panoramic analysis of cell death patterns reveals prognostic and immune profiles of head and neck squamous cell carcinoma

doi: 10.62347/PMDA6193

Figure Lengend Snippet: In immune-competent mouse models, inhibition of CAAP1 induces antitumor immunity. (A) A schematic view of the treatment plan. (B) Tumor bodies dissected 15 days after tumor formation in BLAB/C nude mice. (C, D) Summary of tumor weight (C) and volume data (D) of Fadu tumors harvested after euthanizing the mice. (E) A schematic view of the treatment plan and C57 mice received GFP-Luc sg nc, or sg caap1 meer cells were injected with luciferin. (F, G) Summary of luciferase activity (F) and volume data (G) of meer tumors harvested after euthanizing the mice. (H) Differences in the expression of CAAP1, Bax, and Bak in tumor tissues among different groups. (I-K) FACS of CD8+ in CD3+ cells and GZMB+CD8+ in CD3+ TILs from sg nc, or sg caap1 meer xenografts and quantification.

Techniques: Inhibition, Injection, Luciferase, Activity Assay, Expressing

Journal: American Journal of Cancer Research

Article Title: Panoramic analysis of cell death patterns reveals prognostic and immune profiles of head and neck squamous cell carcinoma

doi: 10.62347/PMDA6193

Techniques: Western Blot, Expressing, Transferring, Knockdown, Migration, Wound Healing Assay, Transwell Assay

Journal: American Journal of Cancer Research

Article Title: Panoramic analysis of cell death patterns reveals prognostic and immune profiles of head and neck squamous cell carcinoma

doi: 10.62347/PMDA6193

Techniques: Cell Counting, CCK-8 Assay, Staining, Flow Cytometry, Western Blot, Enzyme Immunoassay, Control, Activity Assay, Enzymatic Assay

Journal: American Journal of Cancer Research

Article Title: Panoramic analysis of cell death patterns reveals prognostic and immune profiles of head and neck squamous cell carcinoma

doi: 10.62347/PMDA6193

Techniques: Inhibition, Injection, Luciferase, Activity Assay, Expressing

Journal: Cell Journal (Yakhteh)

Article Title: Endoplasmic Reticulum Stress Induces miR-706, A Pro-Cell Death microRNA, in A Protein Kinase RNA-Like ER Kinase (PERK) and Activating Transcription Factor 4 (ATF4) Dependent Manner

doi: 10.22074/cellj.2020.6873

Figure Lengend Snippet: miR-706 tiggers ER stress dependent cell death trough CAAP1. A. Target scan prediction showing predicted binding of miR-706 and CAAP1 3ˊUTR. B. NIH3T3 cells were transfected with scrambled microRNA (control; ctrl) or mimic miR-706 (mimic). Total RNA was purified and CAAP1 expression was determined using qRT-PCR. C. NIH3T3 cells were transduced with antisense miR-706 (anti-706) and then incubated with Thapsigargin for 24 hours. Total RNA was purified and CAAP1 expression was determined using qRT-PCR. D. NIH3T3 cells were transduced with scrambled microRNA (ctrl), antisense miR-706 (anti-706) or antisense miR-706 and shCAAP1. The cells were then incubated with Thapsigargin for 24 hours. Cell death was assesed by Annexin V staining. E. Quantification of panel C. *; P<0.05, **; P<0.01 compared to control, and qRT-PCR; Quantitative reverse transcription polymerase chain reaction.

Article Snippet: The antibodies are: CAAP1 (NBP1-94020, Novus Biologicals, USA) and GAPDH (14C10, Cell Signaling Technology, USA).

Techniques: Binding Assay, Transfection, Control, Purification, Expressing, Quantitative RT-PCR, Transduction, Incubation, Staining, Reverse Transcription, Polymerase Chain Reaction

Figure 1. miR-135a-5p expression is increased in temporal lobe epilepsy. A: Quantitative qRT-PCR analyses of miR-135a-5p levels in hippocampus of children. B: Prediction of miR-135a-5p binding site in Caap1 miRNA 3’-UTR by microRNA website (www.microrna.org/microrna/home.do). C: mRNA level of Caap1 in hippocampus of children. D: Protein abundance of Caap1 in hip-

Journal: Journal of integrative neuroscience

Article Title: Molecular expression and functional analysis of genes in children with temporal lobe epilepsy.

doi: 10.31083/j.jin.2019.01.13

Figure Lengend Snippet: Figure 1. miR-135a-5p expression is increased in temporal lobe epilepsy. A: Quantitative qRT-PCR analyses of miR-135a-5p levels in hippocampus of children. B: Prediction of miR-135a-5p binding site in Caap1 miRNA 3’-UTR by microRNA website (www.microrna.org/microrna/home.do). C: mRNA level of Caap1 in hippocampus of children. D: Protein abundance of Caap1 in hip-

Article Snippet: CAAP1 antibody (NBP1-86644) was obtained from Novus Biologicals (Littleton, CO, USA).

Techniques: Expressing, Quantitative RT-PCR, Binding Assay, Quantitative Proteomics

Figure 4. miR-135a-5p regulates Caap1 expression negatively in TLE model. A: mRNA level of Caap1 in primary rat hippocampus neurons from newborn rats treated with or without miR-135a-5p inhibitor. B: Protein abundance of Caap1 in hippocampus neurons from newborn rats.

Journal: Journal of integrative neuroscience

Article Title: Molecular expression and functional analysis of genes in children with temporal lobe epilepsy.

doi: 10.31083/j.jin.2019.01.13

Figure Lengend Snippet: Figure 4. miR-135a-5p regulates Caap1 expression negatively in TLE model. A: mRNA level of Caap1 in primary rat hippocampus neurons from newborn rats treated with or without miR-135a-5p inhibitor. B: Protein abundance of Caap1 in hippocampus neurons from newborn rats.

Article Snippet: CAAP1 antibody (NBP1-86644) was obtained from Novus Biologicals (Littleton, CO, USA).

Techniques: Expressing, Quantitative Proteomics

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1) Product Images from "Endoplasmic Reticulum Stress Induces miR-706, A Pro-Cell Death microRNA, in A Protein Kinase RNA-Like ER Kinase (PERK) and Activating Transcription Factor 4 (ATF4) Dependent Manner"

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